11/20/2023 0 Comments NucleoplasminWhat portion of the nucleoplasmin molecule is responsible for localization in the nucleus? B. All forms of nucleoplasmin, except heads, accumulated in the nucleus when injected into the cytoplasm, and all forms were retained in the nucleus when injected there. Nucleoplasmin is a heat-stable protein which is able to displace protamines from sperm chromatin. In this experiment, either the intact protein or the nucleoplasmin heads, tails, or heads with a single tail were injected into the cytoplasm of a frog oocyte or into the nucleus. A classic experiment that addressed this problem used several forms of radioactive nucleoplasmin, which is a large pentameric protein involved in chromatin assembly. a membrane-bound nucleus -carbohydrates -a plasma membrane -ribosomes, The cell wall found in bacterial cells functions to. It is easy to obtain an enriched preparation of nucleoplasmin, as it is the major protein remaining soluble after heat denaturation of total egg cytosol (Macaulay & Forbes, 1996). Study with Quizlet and memorize flashcards containing terms like A prokaryotic cell is distinct from a eukaryotic cell because a prokaryotic cell lacks. (A) Purified wild-type nucleoplasmin (Npl) and mutant Y112E or Y124E proteins were added to Xenopus egg extracts with demembraned sperm nuclei to assemble nuclei containing added nucleoplasmin proteins then cytochrome c was added to induce apoptosis. Before nuclear pore complexes were well understood, it was unclear whether nuclear proteins diffused passively into the nucleus and accumulated there by binding to residents of the nucleus such as chromosomes, or whether they were actively imported and accumulated regardless of their affinity for nuclear components. Nucleoplasmin is a heat-stable protein which is able to displace protamines from sperm chromatin. NLS sequences have been identified for many proteins of viral and cellular origins and generally resemble either the single basic-domain SV40 large T-antigen NLS (e.g. Nucleoplasmin-Y124E delayed the occurrence of apoptotic chromatin condensation.
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